MIA 蛋白
Quick Overview for MIA 蛋白 (ABIN2648118)
抗原
See all MIA 蛋白蛋白类型
生物活性
宿主
资源
纯度
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序列
- MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ
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产品特性
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Purified recombinant Human MIA protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 µg/mL. -
内毒素水平
- < 0.1 ng per μg (1 EU/μg).
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Melanoma Inhibitory Activity (MIA) (Active) protein
MIA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Melanoma Inhibitory Activity (MIA) (AA 1-131) protein (GST tag)MIA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Melanoma Inhibitory Activity (MIA) (Transcript Variant 1) (Active) proteinMIA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
Melanoma Inhibitory Activity (MIA) protein (Myc-DYKDDDDK Tag)MIA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Each Investigator should determine their own optimal working dilution for specific applications.
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说明
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Biological activity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 μg/mL.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- Lyophilized from 15 mM Na3PO4, pH 7.2 with 75 mM NaCl.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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- MIA (Melanoma Inhibitory Activity (MIA))
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别名
- MIA
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背景
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MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.
Alternative Names: Cartilage-derived retinoic acid-sensitive protein protein, CD-RAP protein, Melanoma Inhibitory Activity protein -
分子量
- 12.2 kDa
抗原
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