Quick Overview for TM Pst1 蛋白 (ABIN2469152)
抗原
TM Pst1
蛋白类型
Recombinant
宿主
Saccharomyces cerevisiae
资源
Yeast (Saccharomyces Cerevisiae)
应用范围
Screening Assay (ScA), ELISA, Western Blotting (WB)
纯度
~95 %
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应用备注
- This recombinant protein can be used for WB, ELISA, and other assays to screen and identify mannose-specific antibodies.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Lot specific, TBS or PBS
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注意事项
- Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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储存条件
- -80 °C
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储存方法
- Store in working aliquots at -70 °C.
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- TM Pst1
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背景
- Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction (1). Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor (2). Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at around 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (?och1 ?mnn1 ?mnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified glycan-specific PGT antibodies (3). Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows high affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses (4).
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分子量
- 100 kDa on SDS-PAGE
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基因ID
- 851625
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OMIM
- 17380259
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UniProt
- Q12355
抗原
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