The ED50 was determined by the dose - dependent stimulation of the proliferation of Murine FDC - P1 cells is < 0.01 ng/mL, corresponding to a specific activity of > 1.0 x 108 units/mg.
内毒素水平
Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
CSF2
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
注意事项
As with any protein, exposing GM-CSF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
储存条件
-20 °C
储存方法
The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted GM-CSF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant rat GM-CSF is a 14.5 kDa globular protein consisting of 128 amino acids residues.