Biological activity was determined by its ability to inhibit Cathespin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (RLUs). The expected IC50 is = 100nM.
内毒素水平
Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
As with any protein, exposing Fetuin A recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
储存条件
-20 °C
储存方法
The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Fetuin A stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
有效期
24 months
抗原
Fetuin A (AHSG)
(alpha-2-HS-Glycoprotein (AHSG))
别名
Fetuin A
背景
Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-beta and BMP signaling proteins, Recombinant human Fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant Fetuin-A migrates at an apparent molecular weight of 45 - 55 kDa by SDS-PAGE run under non-reducing conditions.