Taq RecA (Active) 蛋白
宿主: Thermus aquaticus
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90 % of protein determined by SDS-PAGE (CBB staining)
Func
Active
1 image
(2 references)-
- 抗原
- Taq RecA
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 宿主
- Thermus aquaticus
- 资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func)
- 产品特性
- Single-strand dependent ATPase activity. The activity of single-stranded DNA-dependent ATPase was confirmed.
- 纯度
- > 90 % of protein determined by SDS-PAGE (CBB staining)
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- 应用备注
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1) Useful for studying homologous recombination
2) Increase the specificity and yield of multiplex PCR (of cDNA or genomic DNA) by promoting homologous annealing of primers to target DNA
3) Visualization of DNA with electoron microscopy due to nucleofilament formation. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 50 mM Tris-HCl ( pH 8.0), 200 mM NaCl, 1 mM EDTA, 50 % glycerol
- 储存条件
- -20 °C
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: "Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products." in: Nucleic acids research, Vol. 33, Issue 14, pp. e126, (2005) (PubMed).
: "The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization." in: Journal of bacteriology, Vol. 176, Issue 5, pp. 1405-12, (1994) (PubMed).
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: "Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products." in: Nucleic acids research, Vol. 33, Issue 14, pp. e126, (2005) (PubMed).
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- 抗原
- Taq RecA
- 背景
- Thermus aquaticus RecA protein is a thermostable enzyme which plays important roles in homologous recombination and DNA repair. This protein has activities of single-stranded DNA dependent ATPase, DNA annealing, and exchanging of strands between two recombining DNA double helices, similar to E.coli RecA protein, but the optimal temperature is between 65~75 °C. Taq RecA was expressed in E.coli in large quantities and the protein was highly purified. MW is 36.5kD.
- UniProt
- P48296
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