Taq RecA (Active) 蛋白
This Recombinant Taq RecA protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 2+ publications.
1 image
(2 references)
产品编号 ABIN2452211
发货至:
中国
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Quick Overview for Taq RecA (Active) 蛋白 (ABIN2452211)
抗原
Taq RecA
蛋白类型
Recombinant
生物活性
Active
宿主
Thermus aquaticus
资源
大肠杆菌（E. Coli）
应用范围
Functional Studies (Func)
纯度
> 90 % of protein determined by SDS-PAGE (CBB staining)
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产品特性
- Single-strand dependent ATPase activity. The activity of single-stranded DNA-dependent ATPase was confirmed.
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应用备注
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1) Useful for studying homologous recombination
2) Increase the specificity and yield of multiplex PCR (of cDNA or genomic DNA) by promoting homologous annealing of primers to target DNA
3) Visualization of DNA with electoron microscopy due to nucleofilament formation. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 50 mM Tris-HCl ( pH 8.0), 200 mM NaCl, 1 mM EDTA, 50 % glycerol
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储存条件
- -20 °C
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: "Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products." in: Nucleic acids research, Vol. 33, Issue 14, pp. e126, (2005) (PubMed).
: "The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization." in: Journal of bacteriology, Vol. 176, Issue 5, pp. 1405-12, (1994) (PubMed).
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: "Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products." in: Nucleic acids research, Vol. 33, Issue 14, pp. e126, (2005) (PubMed).
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- Taq RecA
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背景
- Thermus aquaticus RecA protein is a thermostable enzyme which plays important roles in homologous recombination and DNA repair. This protein has activities of single-stranded DNA dependent ATPase, DNA annealing, and exchanging of strands between two recombining DNA double helices, similar to E.coli RecA protein, but the optimal temperature is between 65~75 °C. Taq RecA was expressed in E.coli in large quantities and the protein was highly purified. MW is 36.5kD.
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UniProt
- P48296
抗原
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