T4 SSB (Active) 蛋白
This Recombinant T4 SSB protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 2+ publications.
(2 references)
产品编号 ABIN2452207
发货至:
中国
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Quick Overview for T4 SSB (Active) 蛋白 (ABIN2452207)
抗原
T4 SSB
蛋白类型
Recombinant
生物活性
Active
宿主
Bacteriophage T4
资源
大肠杆菌（E. Coli）
应用范围
Functional Studies (Func)
纯度
> 95 % of protein determined by SDS-PAGE (CBB staining)
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产品特性
- The absence of endonucleases and exonucleases was confirmed.
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应用备注
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1. Promoting DNA replication and recombination by stabilizing single-stranded DNA
2. Increase specificity and yields of long PCR products -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 10 mg/mL
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缓冲液
- 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).
: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." in: Nature, Vol. 227, Issue 5265, pp. 1313-8, (1970) (PubMed).
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- T4 SSB
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物质类
- Phage Protein
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背景
- T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.
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UniProt
- P03695
抗原
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