HIV1RT 蛋白
This Recombinant HIV1RT protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 3+ publications.
(3 references)Quick Overview for HIV1RT 蛋白 (ABIN2452197)
抗原
HIV1RT
(HIV-1 Reverse Transcriptase (HIV1RT))
蛋白类型
Recombinant
生物活性
Active
宿主
Human Immunodeficiency Virus (HIV)
资源
大肠杆菌（E. Coli）
应用范围
Dot Blot (DB), Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
纯度
> 90 % by SDS-PAGE (CBB staining)
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产品特性
- Activity: 5,000 units/mL
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应用备注
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1) It is extremely effective for screening new specific inhibitors for HIV virus as a drug for treating AIDS.
2) Generally,Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However,by using this enzyme in combination as an antigen,the detection will be more sensitive.
3) Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.
4) Standards for SDS-PAGE -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 50 % glycerol, 40 mM Tris-HCl ( pH 8.3), 50 mM NaCl, 5 mM MgCl2, 0.1 % Triton X-100, 1 mM DTT
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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: "Overproduction of human immunodeficiency virus type I reverse transcriptase in Escherichia coli and purification of the enzyme." in: Microbiology and immunology, Vol. 34, Issue 6, pp. 509-21, (1990) (PubMed).
: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).
: "[Considerations on antacid therapy]." in: Medizinische Klinik, Vol. 72, Issue 30, pp. 1229-41, (1977) (PubMed).
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: "Overproduction of human immunodeficiency virus type I reverse transcriptase in Escherichia coli and purification of the enzyme." in: Microbiology and immunology, Vol. 34, Issue 6, pp. 509-21, (1990) (PubMed).
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- HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))
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别名
- HIV-1 Reverse Transcriptase
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物质类
- Viral Protein
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背景
- HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase derived from HIV-1 (AIDS virus), subtype B origin. It also has RNaseH activity and is an enzyme indispensable for reproduction of AIDS virus. This protein is uniquely over-expressed as a recombinant protein in E. coli by a patented method and highly purified. It is composed of two subunits whose molecular weights are 66 kD and 51 kD, same as the those of the enzyme purified from AIDS virus particles.
抗原
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