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HIV-1 Gag P15 蛋白

宿主: Human Immunodeficiency Virus (HIV) 宿主: 大肠杆菌(E. Coli) Recombinant > 90 % by SDS-PAGE (CBB staining) DB, SDS, ELISA, WB
产品编号 ABIN2452192
发货至: 中国
  • 抗原
    HIV-1 Gag P15
    蛋白类型
    Recombinant
    宿主
    Human Immunodeficiency Virus (HIV)
    资源
    大肠杆菌(E. Coli)
    应用范围
    Dot Blot (DB), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
    纯度
    > 90 % by SDS-PAGE (CBB staining)
  • 应用备注
    1) It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.
    2) It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.
    3) It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.
    4) It can be used as a standard for the quantitative analysis of HIV-1 p15 antigen.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.42 mg/mL
    缓冲液
    50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
    储存条件
    -20 °C
  • Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).

    Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).

    Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

  • 抗原
    HIV-1 Gag P15
    物质类
    Viral Protein
    背景
    HIV-1 Gag p15 is processed by digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA. HIV-1 Gag p15 was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, subtype B, and highly purified by several steps of chromatography. Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles.
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