RuvC 蛋白
This Native RuvC protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 3+ publications.
(3 references)
产品编号 ABIN2452186
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for RuvC 蛋白 (ABIN2452186)
抗原
RuvC
(Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
蛋白类型
Native
生物活性
Active
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
-
-
产品特性
- Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
-
-
Crossover Junction Endodeoxyribonuclease RuvC (RuvC) (AA 2-173) protein (His-SUMO Tag)
RuvC 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
-
-
应用备注
-
1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
2) SDS-PAGE (0. 2 g/lane)
3) Standard antigen for western blotting and ELISA -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
- 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
-
储存条件
- -20 °C/-80 °C
-
储存方法
- Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
-
-
-
: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).
: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).
: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." in: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, (1992) (PubMed).
-
: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).
-
- RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
-
别名
- RuvC
-
背景
- E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
-
UniProt
- P0A814
抗原
-
You are here:
