RuvC 蛋白
This Native RuvC protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 3+ publications.
(3 references)Quick Overview for RuvC 蛋白 (ABIN2452186)
抗原
RuvC
(Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
蛋白类型
Native
生物活性
Active
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
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产品特性
- Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
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Crossover Junction Endodeoxyribonuclease RuvC (RuvC) (AA 2-173) protein (His-SUMO Tag)
RuvC 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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应用备注
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1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
2) SDS-PAGE (0. 2 g/lane)
3) Standard antigen for western blotting and ELISA -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
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储存条件
- -20 °C/-80 °C
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储存方法
- Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
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: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).
: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).
: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." in: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, (1992) (PubMed).
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: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).
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- RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
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别名
- RuvC
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背景
- E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
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UniProt
- P0A814
抗原
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