RuvB (Active) 蛋白

ABIN2452184 产品详细信息, 供应商: Log in to see
大肠杆菌(E. Coli)
大肠杆菌(E. Coli)
Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
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产品特性 Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvB protein is over 90 % by SDS-PAGE (CBB staining) pure.
背景 E. coli RuvB protein forms a complex with RuvA protein at the late stage of homologous recombination and recombination repair and binds specifically to the Holliday structure which is the intermediate of recombination, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. RuvB forms a hexamer ring structure and surrounds the double chain DNA and covers RuvA tetramer bound to the Holliday junction from both sides. RuvB is a DNA motor protein which possesses the ATPase activity, activated by DNA and RuvA protein. Its molecular weight is 37 kD and forms a dimer in solution in the physiological condition. .
UniProt P0A812
应用备注 1) Functional studies in vitro,RuvA and RuvB form a complex that promotes Holiday junction (a recombination intermediatae) branch-migration by using ATP hydrolysis energy. RuvB also has ATPase activity which is stimulated by RuvA and DNA.
2) SDS-PAGE (0. 2 g/lane)
3) Standard antigen for western blotting and ELISA
限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL
缓冲液 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
储存条件 -20 °C/-80 °C
储存方法 Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
SDS-PAGE (SDS) image for RuvB (Active) protein (ABIN2452184) RuvB (Active) protein
SDS-PAGE (SDS) image for RuvB (Active) protein (ABIN2452184) RuvB (Active) protein (Image 2)
SDS-PAGE (SDS) image for RuvB (Active) protein (ABIN2452184) RuvB (Active) protein (Image 3)
有引用在: Shinagawa, Iwasaki: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, 1996 (PubMed).

Iwasaki, Takahagi, Nakata, Shinagawa: "Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration." in: Genes & development, Vol. 6, Issue 11, pp. 2214-20, 1992 (PubMed).