RuvB (Active) 蛋白
This Native protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 2+ publications.
(2 references)Quick Overview for RuvB (Active) 蛋白 (ABIN2452184)
抗原
RuvB
蛋白类型
Native
生物活性
Active
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
-
-
产品特性
- Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvB protein is over 90 % by SDS-PAGE (CBB staining) pure.
-
-
想要此Protein的其他选项吗？
!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 RabbitConjugateOrigin Discosoma价格从 3,390.66 ¥表达系统 Guinea PigConjugateOrigin Rabbit价格从 3,390.66 ¥表达系统 RabbitConjugateOrigin Cow, Human, Mouse, Pig, Rabbit, Rat价格从 1,093.86 ¥表达系统 RabbitConjugateOrigin Cow, Human, Mouse, Pig, Rat价格从 5,693.28 ¥表达系统 RabbitConjugateOrigin Human, Monkey, Mouse, Pig, Rat, Sheep价格从 3,240.03 ¥表达系统 RabbitConjugateOrigin Horse, Human, Mouse, Rabbit, Rat价格从 3,240.03 ¥表达系统 RabbitConjugateOrigin Human, Mouse, Rat价格从 3,240.03 ¥表达系统 GoatConjugateOrigin Aequorea victoria价格从 3,390.66 ¥表达系统 MouseConjugateOrigin Human价格从 6,107.06 ¥表达系统ConjugateOrigin Rat价格从 4,655.83 ¥
5,477.45 ¥-15%您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
-
RuvB (AA 1-336), (full length) protein (His-SUMO Tag)
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
-
-
应用备注
-
1) Functional studies in vitro,RuvA and RuvB form a complex that promotes Holiday junction (a recombination intermediatae) branch-migration by using ATP hydrolysis energy. RuvB also has ATPase activity which is stimulated by RuvA and DNA.
2) SDS-PAGE (0. 2 g/lane)
3) Standard antigen for western blotting and ELISA -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
- 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
-
储存条件
- -20 °C/-80 °C
-
储存方法
- Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
-
-
-
: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).
: "Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration." in: Genes & development, Vol. 6, Issue 11, pp. 2214-20, (1992) (PubMed).
-
: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).
-
- RuvB
-
背景
- E. coli RuvB protein forms a complex with RuvA protein at the late stage of homologous recombination and recombination repair and binds specifically to the Holliday structure which is the intermediate of recombination, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. RuvB forms a hexamer ring structure and surrounds the double chain DNA and covers RuvA tetramer bound to the Holliday junction from both sides. RuvB is a DNA motor protein which possesses the ATPase activity, activated by DNA and RuvA protein. Its molecular weight is 37 kD and forms a dimer in solution in the physiological condition. .
-
UniProt
- P0A812
抗原
-
You are here:
