RuvA 蛋白
This Recombinant RuvA protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 2+ publications.
(2 references)
产品编号 ABIN2452182
发货至:
中国
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Quick Overview for RuvA 蛋白 (ABIN2452182)
抗原
RuvA
蛋白类型
Recombinant
生物活性
Active
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
Functional Studies (Func)
纯度
> 90 % by SDS-PAGE (CBB staining)
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产品特性
- The product is a recombinant protein abundantly expressed by E. coli and purified by methods such as chromatography. The molecular weight is 22 kD and even in solution, it binds to the Holliday structure and form a tetramer.
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RuvA (AA 1-203), (full length) protein (His-SUMO Tag)
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
RuvA (Active) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Native Func Active
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应用备注
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1) Functional as Holliday junction specific binding protein,which promotes Holliday-junction branch migration in combination with RuvB protein.
2) For SNP analysis (Genome Research 13:1754-1764 PMID: 12840050). -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.7 mg/mL
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缓冲液
- 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
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储存条件
- -20 °C
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: "Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 31, pp. 11544-8, (2006) (PubMed).
: "Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration." in: Genes & development, Vol. 6, Issue 11, pp. 2214-20, (1992) (PubMed).
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: "Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 31, pp. 11544-8, (2006) (PubMed).
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- RuvA
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背景
- E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.
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UniProt
- P0A809
抗原
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