RecA (Active) 蛋白
宿主: E. coli
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90 % by SDS-PAGE (CBB staining)
Func
Active
(2 references)-
- 抗原
- RecA
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 宿主
- E. coli
- 资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func)
- 产品特性
- The product is over-expressed as a recombinant protein and highly purified by several steps of chromatography. A single band is observed by SDS-PAGE at 38 kD.
- 纯度
- > 90 % by SDS-PAGE (CBB staining)
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- 应用备注
-
1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization (2).
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.6 mg/mL
- 缓冲液
- 20 mM Tris-HCl ( pH 8.0), 1 mM EDTA, 150 mM KCl, 1 mM DTT, 50 % glycerol
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20 C (For long term storage: -70 C)
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-
: "Understanding the complexity of an organism's responses to DNA damage." in: Cold Spring Harbor symposia on quantitative biology, Vol. 65, pp. 1-10, (2003) (PubMed).
: "Use of RecA protein to enrich for homologous genes in a genomic library." in: Nucleic acids research, Vol. 16, Issue 16, pp. 8157-69, (1988) (PubMed).
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: "Understanding the complexity of an organism's responses to DNA damage." in: Cold Spring Harbor symposia on quantitative biology, Vol. 65, pp. 1-10, (2003) (PubMed).
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- 抗原
- RecA
- 背景
- E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loop and Holliday structure in homologous recombination reaction, and coprotease activities that promote self-cleavages of LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its homologs in eukaryotes are Rad51 protein and Dmcl protein.
- UniProt
- P0A7G6
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