RNASEH 蛋白
This Recombinant RNASEH protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 3+ publications.
1 image
(3 references)
产品编号 ABIN3200996
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for RNASEH 蛋白 (ABIN3200996)
抗原
See all RNASEH 蛋白
RNASEH
(Ribonuclease H (RNASEH))
蛋白类型
Recombinant
生物活性
Active
宿主
E. coli
资源
大肠杆菌（E. Coli）
纯度
> 95 % protein determined by SDS-PAGE (CBB staining)
-
-
产品特性
-
Specific Activity: 100, 000 units/mg protein
Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37 C.
-
-
-
-
应用备注
-
1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA.
2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT)
3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 50 units/L
-
缓冲液
- 50 units/ul in 20 mM Tris-HCl ( pH 7.5), 100 mM KCl, 1 mM DTT, 50 % Glycerol
-
储存液
- Dithiothreitol (DTT)
-
注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
-
-
: "Second-strand cDNA synthesis: mRNA fragments as primers." in: Methods in enzymology, Vol. 152, pp. 330-5, (1987) (PubMed).
: "Site specific enzymatic cleavage of RNA." in: Nucleic acids research, Vol. 7, Issue 1, pp. 179-92, (1979) (PubMed).
: "Electrophoretic patterns of deadenylylated chorion and globin mRNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 8, pp. 2959-63, (1976) (PubMed).
-
: "Second-strand cDNA synthesis: mRNA fragments as primers." in: Methods in enzymology, Vol. 152, pp. 330-5, (1987) (PubMed).
-
- RNASEH (Ribonuclease H (RNASEH))
-
背景
- Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.
-
UniProt
- P0A7Y4
抗原
-
You are here:
