Botulinum C3 Enzyme (Active) 蛋白
宿主: Clostridium botulinum
宿主: Clostridium botulinum
Native
> 90 % purity by SDS-PAGE (CBB staining)
Func
Active
1 image
(2 references)-
- 抗原
- Botulinum C3 Enzyme
- 蛋白类型
- Native
- 生物活性
- Active
- 宿主
- Clostridium botulinum
- 资源
- Clostridium botulinum
- 应用范围
- Functional Studies (Func)
- 纯度
- > 90 % purity by SDS-PAGE (CBB staining)
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- 应用备注
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1) For the studies on the Rho-dependent signaling pathways in animal cells in vivo and in vitro.
2) ADP-ribosylation of small GTP proteins, such as Rho and Rac proteins.
Activity: Stimulation of human keratinocytes in culture was observed at 50 ng/mL. - 说明
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No toxic effect was observed in two mice injected with 5 ug of C3 enzyme, showing little contamination of lethal neurotoxins from the botulinus culuture in this preparation.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.33 mg/mL
- 缓冲液
- 5 mM sodium phosphate buffer ( pH 6.0), 50 % glycerol
- 储存条件
- -20 °C/-80 °C
- 储存方法
- -20 C (For long term storage: -70 C)
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: "Bacterial toxins and the Rho GTP-binding protein: what microbes teach us about cell regulation." in: Cell death and differentiation, Vol. 5, Issue 9, pp. 720-8, (1999) (PubMed).
: "Purification and characterization of ADP-ribosyltransferases (exoenzyme C3) of Clostridium botulinum type C and D strains." in: Journal of bacteriology, Vol. 173, Issue 19, pp. 6025-9, (1991) (PubMed).
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: "Bacterial toxins and the Rho GTP-binding protein: what microbes teach us about cell regulation." in: Cell death and differentiation, Vol. 5, Issue 9, pp. 720-8, (1999) (PubMed).
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- 抗原
- Botulinum C3 Enzyme
- 背景
- This product was highly purified from the supernatant of the culture of Clostridium botulinum type C by cation exchange, hydroxyapatite and gel filtration columns. This toxin ADP-ribosylates Rho and Rac, which are low-molecular weight GTP-binding proteins of animal cells, and interrupts the downstream signal transduction pathways. The molecular weight is 24 kDa.
- UniProt
- P15879
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