VEGF-A121 (AA 28-147) (Active) 蛋白
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for VEGF-A121 (AA 28-147) (Active) 蛋白 (ABIN2018455)
抗原
VEGF-A121
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 % as analyzed by SDS-PAGE and HPLC.
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产品特性
- AA 28-147
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产品特性
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ED50 < 5 ng/mL, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2x 10^5 units/mg.
AA 28-147, expressed with an N-terminal Met. -
内毒素水平
- < 0.2 EU/μg, determined by LAL method.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstituted in ddH2O or PBS at 100 μg/mL.
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缓冲液
- Lyophilized after extensive dialysis against PBS.
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储存条件
- -80 °C
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储存方法
- Lyophilized recombinant human VEGF-A121 (rhVEGF-A121) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhVEGF-A121 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
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有效期
- 6 months
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- VEGF-A121
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背景
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VEGF-A121 is one of five isoforms (121, 145, 165, 189, and 206) of VEGF protein, a cytokine belonging to the Platelet Differentiation Growth Factor (PDGF) family, and existing as a disulfide-linked homodimeric glycoprotein. In contrast to the longer isoforms, VEGF-A121 is more freely diffusible, and cannot bind to heparin. In vivo, VEGF is expressed predominantly in lung, heart, kidney, and adrenal glands, and the expression of VEGF is up-regulated by a number of growth factors, including PDGF, Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Tumor Necrosis Factor (TNF). VEGF signals via binding to two tyrosine kinase receptors: the Fms-like tyrosine kinase 1 (Flt-1) and the kinase domain receptor (KDR). VEGF is a specific mitogen and survival factor, contributing to abnormal angiogenesis and cancer development. Recombinant human VEGF-A121 (rhVEGF-A121) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 121 amino acids each. A fully biologically active molecule, rhVEGF-A121 has a molecular mass of 28.2 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: Vascular Endothelial Growth Factor121, VPF -
分子量
- 28.2 kDa, observed by non-reducing SDS-PAGE.
抗原
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