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VEGF-A121 (AA 28-147) (Active) 蛋白

宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
产品编号 ABIN2018455
发货至: 中国
  • 抗原
    VEGF-A121
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 28-147
    宿主
    资源
    • 1
    大肠杆菌(E. Coli)
    产品特性
    ED50 < 5 ng/mL, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2x 10^5 units/mg.
    AA 28-147, expressed with an N-terminal Met.
    纯度
    > 95 % as analyzed by SDS-PAGE and HPLC.
    内毒素水平
    < 0.2 EU/μg, determined by LAL method.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstituted in ddH2O or PBS at 100 μg/mL.
    缓冲液
    Lyophilized after extensive dialysis against PBS.
    储存条件
    -80 °C
    储存方法
    Lyophilized recombinant human VEGF-A121 (rhVEGF-A121) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhVEGF-A121 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
    有效期
    6 months
  • 抗原
    VEGF-A121
    背景
    VEGF-A121 is one of five isoforms (121, 145, 165, 189, and 206) of VEGF protein, a cytokine belonging to the Platelet Differentiation Growth Factor (PDGF) family, and existing as a disulfide-linked homodimeric glycoprotein. In contrast to the longer isoforms, VEGF-A121 is more freely diffusible, and cannot bind to heparin. In vivo, VEGF is expressed predominantly in lung, heart, kidney, and adrenal glands, and the expression of VEGF is up-regulated by a number of growth factors, including PDGF, Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Tumor Necrosis Factor (TNF). VEGF signals via binding to two tyrosine kinase receptors: the Fms-like tyrosine kinase 1 (Flt-1) and the kinase domain receptor (KDR). VEGF is a specific mitogen and survival factor, contributing to abnormal angiogenesis and cancer development. Recombinant human VEGF-A121 (rhVEGF-A121) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 121 amino acids each. A fully biologically active molecule, rhVEGF-A121 has a molecular mass of 28.2 kDa analyzed by non-reducing SDS-PAGE.
    Synonyms: Vascular Endothelial Growth Factor121, VPF
    分子量
    28.2 kDa, observed by non-reducing SDS-PAGE.
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