VEGF-A121 (AA 28-147) (Active) 蛋白
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % as analyzed by SDS-PAGE and HPLC.
Active
-
- 抗原
- VEGF-A121
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 28-147
- 宿主
- 人
- 资源
- 大肠杆菌（E. Coli）
- 产品特性
-
ED50 < 5 ng/mL, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2x 10^5 units/mg.
AA 28-147, expressed with an N-terminal Met. - 纯度
- > 95 % as analyzed by SDS-PAGE and HPLC.
- 内毒素水平
- < 0.2 EU/μg, determined by LAL method.
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstituted in ddH2O or PBS at 100 μg/mL.
- 缓冲液
- Lyophilized after extensive dialysis against PBS.
- 储存条件
- -80 °C
- 储存方法
- Lyophilized recombinant human VEGF-A121 (rhVEGF-A121) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhVEGF-A121 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- 有效期
- 6 months
-
- 抗原
- VEGF-A121
- 背景
-
VEGF-A121 is one of five isoforms (121, 145, 165, 189, and 206) of VEGF protein, a cytokine belonging to the Platelet Differentiation Growth Factor (PDGF) family, and existing as a disulfide-linked homodimeric glycoprotein. In contrast to the longer isoforms, VEGF-A121 is more freely diffusible, and cannot bind to heparin. In vivo, VEGF is expressed predominantly in lung, heart, kidney, and adrenal glands, and the expression of VEGF is up-regulated by a number of growth factors, including PDGF, Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Tumor Necrosis Factor (TNF). VEGF signals via binding to two tyrosine kinase receptors: the Fms-like tyrosine kinase 1 (Flt-1) and the kinase domain receptor (KDR). VEGF is a specific mitogen and survival factor, contributing to abnormal angiogenesis and cancer development. Recombinant human VEGF-A121 (rhVEGF-A121) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 121 amino acids each. A fully biologically active molecule, rhVEGF-A121 has a molecular mass of 28.2 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: Vascular Endothelial Growth Factor121, VPF - 分子量
- 28.2 kDa, observed by non-reducing SDS-PAGE.
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