PDGF-BB Homodimer (AA 74-182) (Active) 蛋白
Quick Overview for PDGF-BB Homodimer (AA 74-182) (Active) 蛋白 (ABIN2018324)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 74-182
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产品特性
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ED50 < 2 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5 x 10^5 units/mg.
AA 74-182, expressed with an N-terminal Met. -
内毒素水平
- < 0.2 EU/μg, determined by LAL method.
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PDGF-BB Homodimer (Active) protein (His tag)
宿主: 人 宿主: 酵母菌 Recombinant > 95 % as determined by reducing SDS-PAGE. Active
PDGF-BB Homodimer protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 98 % as determined by reducing SDS-PAGE.
PDGF-BB Homodimer (AA 82-190) (Active) protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by reducing (R) and non-reducing (NR) SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstituted in 20 mM acetic acid at 100 μg/mL.
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缓冲液
- Lyophilized after extensive dialysis against 20 mM acetic acid.
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储存条件
- -80 °C
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储存方法
- Lyophilized recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrPDGF-BB remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
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有效期
- 6 months
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- PDGF-BB Homodimer
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别名
- Platelet-Derived Growth Factor-BB (PDGF-BB)
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背景
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Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rrPDGF-BB has a molecular mass of 24.6 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: Platelet-Derived Growth Factor-BB, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF) -
分子量
- 24.6 kDa, observed by non-reducing SDS-PAGE.
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UniProt
- Q05028
抗原
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