Long R3 Insulin Like Growth Factor-1 (Active) 蛋白
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for Long R3 Insulin Like Growth Factor-1 (Active) 蛋白 (ABIN2018201)
抗原
Long R3 Insulin Like Growth Factor-1
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 % by SDS-PAGE.
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序列
- MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
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产品特性
- ED50 < 10 ng/mL, measured by the dose-dependantproliferation of CHO cells, corresponding to a specific activity of >1.0x 10^5 units/mg.
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内毒素水平
- < 1 EU/μg, determined by LAL method.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstituted in 10 mM HCl at 1 mg/mL. Use a 0.22 μm membrane for filter Sterilization.
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缓冲液
- Lyophilized after dissolved in 48 % acetonitrile and 0.1 % TFA
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Lyophilized recombinant human Long R3Insulin-like Growth Factor-1 (rhLR3IGF-1) remains stable up to 6 months at -80 °C from date of receipt. Stock solution of peptide can be stored at least 3 months at -20 °C.
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有效期
- 6 months
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- Long R3 Insulin Like Growth Factor-1
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背景
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IGF-1 is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-1 is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-2, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-1 (rhLR3IGF-1) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3)and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs. RhLR3IGF-1 is properly folded under oxidizing conditions and then purified by proprietary chromatographic techniques.
Synonyms: Human LR3 Insulin-like Growth Factor-I (LR3-IGF-I) (Receptor Grade) -
分子量
- 9.1±0.9 KDa, observed by reducing SDS-PAGE.
抗原
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