LIF Protein (AA 23-202)
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Quick Overview for LIF Protein (AA 23-202) (ABIN2018195)
抗原
See all LIF 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 23-202
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产品特性
- ED50 < 0.2 ng/mL, measured by a cell differentiation assay using TF-I cells, corresponding to a specific activity of > 5 x 10^6 units/mg.
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内毒素水平
- < 0.2 EU/μg, determined by LAL method.
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Leukemia Inhibitory Factor (LIF) (AA 23-202) protein
LIF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) protein (Fc Tag)LIF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) protein (His tag)LIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (GST tag)LIF 宿主: 人, 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % (SDS-PAGE) SDS Active
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (Fc Tag)LIF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) protein (His tag)LIF 宿主: 小鼠 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstituted in ddH2O at 100 μg/mL.
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缓冲液
- Lyophilized after extensive dialysis against PBS.
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储存条件
- -80 °C
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储存方法
- Lyophilized recombinant human Leukemia Inhibitory Factor (rhLIF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhLIF remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C. ,
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有效期
- 6 months
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: "A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under chemically defined conditions of dual kinase inhibition." in: PLoS ONE, Vol. 6, Issue 9, pp. e24501, (2011) (PubMed).
: "TNF reduces LIF endocytosis despite increasing NFkappaB-mediated gp130 expression." in: Journal of cellular physiology, Vol. 213, Issue 1, pp. 161-6, (2007) (PubMed).
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: "A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under chemically defined conditions of dual kinase inhibition." in: PLoS ONE, Vol. 6, Issue 9, pp. e24501, (2011) (PubMed).
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- LIF (Leukemia Inhibitory Factor (LIF))
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背景
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Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low, and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.Recombinant human Leukemia Inhibitory Factor (rhLIF) produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rhLIF has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: D factor, MLPLI -
分子量
- 19.7 kDa, observed by reducing SDS-PAGE.
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UniProt
- P15018
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
抗原
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