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TMPRSS15 蛋白

This Recombinant TMPRSS15 protein is expressed in Yeast (Pichia pastoris).
产品编号 ABIN2017742
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for TMPRSS15 蛋白 (ABIN2017742)

抗原

See all TMPRSS15 蛋白
TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

蛋白类型

Recombinant

宿主

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Cow

资源

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Yeast (Pichia pastoris)

纯度

> 95 % by SDS-PAGE analyses.
  • 产品特性

    5 IU/μl
    Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 22 °C in a buffer containing 25 mM Tris-HCl, pH 8.0.

    过滤

    Sterile

    内毒素水平

    < 1.0 EU/μg, determined by LAL method.

    组件

    100 IU (or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
    100 μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
    3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
    1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl2) Storage: Store kit components at -20 °C.
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  • 限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Sterile liquid solution contians 20 mM Tris, 200 mM NaCl, 2 mM CaCl2, 50 % glycerol, pH 7.4.

    注意事项

    Avoid freeze-thaw cycles.

    储存条件

    -20 °C

    储存方法

    Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20 °C from date of receipt. It will remain stable at 37 °C for one week without losing any activity.

    有效期

    12 months
  • 抗原

    TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

    别名

    Enterokinase(EK)

    背景

    Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques.
    Synonyms: Enteropeptidase, ENTK, PRSS7

    分子量

    Theoretical MW: 22.7 kDa.
    Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.
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