TMPRSS15 蛋白
This Recombinant TMPRSS15 protein is expressed in Yeast (Pichia pastoris).
Quick Overview for TMPRSS15 蛋白 (ABIN2017742)
抗原
See all TMPRSS15 蛋白
TMPRSS15
(Transmembrane Protease, serine 15 (TMPRSS15))
蛋白类型
Recombinant
宿主
Cow
资源
Yeast (Pichia pastoris)
纯度
> 95 % by SDS-PAGE analyses.
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产品特性
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5 IU/μl
Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 22 °C in a buffer containing 25 mM Tris-HCl, pH 8.0. -
过滤
- Sterile
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内毒素水平
- < 1.0 EU/μg, determined by LAL method.
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组件
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100 IU (or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
100 μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl2) Storage: Store kit components at -20 °C.
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Transmembrane Protease, serine 15 (TMPRSS15) (AA 785-10199) protein
TMPRSS15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Sterile liquid solution contians 20 mM Tris, 200 mM NaCl, 2 mM CaCl2, 50 % glycerol, pH 7.4.
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注意事项
- Avoid freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20 °C from date of receipt. It will remain stable at 37 °C for one week without losing any activity.
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有效期
- 12 months
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- TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))
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别名
- Enterokinase(EK)
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背景
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Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques.
Synonyms: Enteropeptidase, ENTK, PRSS7 -
分子量
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Theoretical MW: 22.7 kDa.
Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.
抗原
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