Quick Overview for CDK5R1 蛋白 (ABIN1686721)
抗原
See all CDK5R1 蛋白蛋白类型
宿主
资源
应用范围
纯度
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序列
- SHMQPASAKW YDRRDYVFIE FCVEDSKDVN VNFEKSKLTF SCLGGSDNFK HLNEIDLFHC IDPNDSKHKR TDRSILCCLR KGESGQSWPR LTKERAKLNW LSVDFNNWKD WEDDSDEDMS NFDRFSEMMN NMGGDEDVDL PEVDGADDDS QDSDDEKMPD LE
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特异性
- ~23 kDa
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产品特性
- 4 µM ABIN1686720, when added to 2 µM SPR-300 (Aha1)-activated Hsp90 (2 µM, His-tagged Hsp90 beta) in 33 mM Hepes pH 7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µL reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
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纯化方法
- Affinity Purified
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Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1) (AA 1-160) protein (His tag)
CDK5R1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1) (AA 208-307) protein (GST tag)CDK5R1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1) (AA 1-307) protein (GST tag)CDK5R1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product has been certified >90% pure using SDS PAGE analysis. 4uM ABIN1686720, when added to 2uM SPR-300 (Aha1)-activated HSP90 (2uM, His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
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限制
- 仅限研究用
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浓度
- Lot specific
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缓冲液
- 20 mM HEPES buffer pH 7.2, 80 mM NaCl, 10 % glycerol
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储存条件
- -20 °C
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- CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))
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别名
- p23
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物质类
- Viral Protein
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背景
- P23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the HSP90-progesterone receptor complex along with HSP70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate-mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.
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分子量
- approx. 23 kDa
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基因ID
- 10728
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NCBI登录号
- NP_006592
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UniProt
- Q15185
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途径
- Stem Cell Maintenance, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
抗原
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