Optimized DNA sequence encoding extracellular domain of mouse CD66a including a C-terminal 6His tag was expressed in HEK293 cells.
产品特性
Recombinant mouse CD66a (CEACAM-1) is a monomer protein consisting of 405 amino acid residue subunits, due to glycosylation migrates as an approximately 65-85 kDa protein on SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
CEACAM1
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
> 90 % as determined by reducing SDS-PAGE.
Imm
限制
仅限研究用
缓冲液
PBS solution, pH7.2
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
-20 °C
储存方法
The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.
Stem Cell Factor is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and earlydifferentiation of cells at the level of multipotential stem cells. It has been suggested thatSCF is essential for optimal production of various hematopoietic lineages, mainly becauseof its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR (CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.