CD34 Protein (CD34) (C-Term, Extracellular Domain)
Quick Overview for CD34 Protein (CD34) (C-Term, Extracellular Domain) (ABIN1684672)
抗原
See all CD34 蛋白蛋白类型
生物活性
宿主
资源
纯度
-
-
产品特性
- C-Term, Extracellular Domain
-
特异性
- Optimized DNA sequence encoding extracellular domain of mouse CD34 including a C-terminal 6His tag was expressed in HEK293 cells.
-
产品特性
- Recombinant mouse CD34 is a monomer protein consisting of70 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
-
-
CD34 (CD34) (AA 32-290) protein (His tag)
CD34 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD34 (CD34) (AA 35-287) protein (His tag)CD34 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD34 (CD34) (AA 124-225) protein (His tag)CD34 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
CD34 (CD34) (AA 32-141) protein (GST tag)CD34 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
CD34 (CD34) (AA 1-328) protein (GST tag)CD34 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
CD34 (CD34) protein (Fc Tag)CD34 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
CD34 (CD34) (AA 32-290) protein (His tag)CD34 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
CD34 (CD34) (Active) protein (His tag)CD34 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
CD34 (CD34) (AA 35-287) protein (Fc Tag)CD34 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
-
-
说明
-
Biologically active: Measured by the ability to support the adhesion of the HUVEC cell line. Coated plates with immobilized recombinant mouse CD34 ( 0.8 µg/mL) are able to adhere 40 % of cells ( 4 x 104 cells/well) after one hour at 37 °C
-
限制
- 仅限研究用
-
-
-
溶解方式
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
-
缓冲液
- PBS solution, pH7.2.
-
注意事项
- Avoid repeated freeze/thaw cycles.
-
储存条件
- -20 °C
-
储存方法
- The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.
-
有效期
- 12-24 months
-
-
- CD34
-
别名
- CD34
-
背景
- Parathyroid hormone is a circulating hormone that acts as the central regulator of calcium metabolism by directly targeting bone, kidney, and intestine. The classical concept of PTH action is that it regulates serum calcium levels by stimulating bone resorption, however, intermittent administration of PTH selectively stimulates bone formation. It is now known that PTH binds to its receptor PTH1R, and activates the G protein alpha subunits. Besides PKA and PKC activation, PTH also regulates MAPKs including p42/p44 ERKs, p38, and c-Jun N-terminal kinase subtypes.
-
UniProt
- Q64314
抗原
-
