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CD200 Protein (CD200) (C-Term, Extracellular Domain)

This Recombinant CD200 protein is expressed in HEK-293T Cells.
产品编号 ABIN1684663
发货至: 中国

Quick Overview for CD200 Protein (CD200) (C-Term, Extracellular Domain) (ABIN1684663)

抗原

See all CD200 蛋白
CD200

蛋白类型

Recombinant

生物活性

Active

宿主

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小鼠

资源

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HEK-293T Cells

纯度

> 98 %, as determined by SDS-PAGE and HPLC
  • 产品特性

    C-Term, Extracellular Domain

    特异性

    Optimized DNA sequence encoding extracellular domain of Mouse CD200 including a C-terminal 6His tag was expressed in HEK293 cells.

    产品特性

    Recombinant Mouse CD200 is a monomer protein consisting of13 amino acid residue subunits, due to glycosylation migrates as an approximately 40-45kDa protein on SDS-PAGE.

    过滤

    0.2 μm filtered

    内毒素水平

    Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
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  • 说明

    The activity was tested by the ability of immobilized recombinant mouse CD200 (1 µg/mL,100 µL/well) to bind mouse CD200R1 with a linear range of 1-200 ng/mL

    限制

    仅限研究用
  • 缓冲液

    PBS solution, pH7.2

    注意事项

    Avoid repeated freeze/thaw cycles.

    储存条件

    -20 °C

    储存方法

    The lyophilized antibody is stable for at least 1 year from date of receipt at -20 °C. Upon reconstitution, this antibody can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months without detectable loss of activity.

    有效期

    12 months
  • 抗原

    CD200

    别名

    CD200

    背景

    CD7 is a 40-kD member of the Ig gene superfamily that is expressed on a major subset of human peripheral T lymphocytes and NK cells. CD7 can complex with CD3 and CD45 molecules, and CD7 signaling involves both protein kinase C and protein tyrosine kinase. CD7 has been shown to be a functional signal-transducing molecule on resting NK cells. Although the function of CD7 is unknown, studies suggest that CD7 may be involved in T and NK cell activation and/or adhesion. In TCRbeta T cells, cross-linking of CD7 leads to rapid increases in intracellular calcium concentrations, induces CD7 cytoplasmic domain association with phosphotidylinositol-3-kinase, synergizes with submitogenic amounts of CD3 mAbs to induce T cell activation, modulates T cell adhesion, and increases T cell IL-2 production and IL-2 receptor expression. In TCR T cells, cross-linking of CD7 leads to cell activation and induction of TNF-a, TNF-b, and granulocyte-macrophage CSF mRNA.

    UniProt

    O54901
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