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- 抗原 See all HCP products
- HCP (Host Cell Proteins (HCP))
- 蛋白类型
- Native
- 宿主
- E. coli
- 资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Western Blotting (WB)
- 原理
- E.coli Host Cell Protein Control Protein (HCP) (in 1x Loading Buffer)
- 特异性
- E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E. coli (BL21) cells.
- 产品特性
- Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: E.coli Host Cell Proteins , HCP antigen, E.coli control antigen
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- 应用备注
- Western Blot Dilution: 1:500 - 1:1000
- 说明
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E. coli Host Cell Protein Control Proteins detects over 300 E.coli proteins and is suitable for western blotting and ELISA. Kits are also useful in quality control applications as well as lot release testing. Regulations require HCPs be reduced to the lowest levels possible. Our generic ELISA kits are useful in both process development and process validation. Using these kits in the earliest phase of product development can provide relevant and specific data directing process improvement and decreasing the levels of HCP contamination.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 4.0 mg/mL
- 缓冲液
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
- Store Host Cell Proteins control protein at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- HCP (Host Cell Proteins (HCP))
- 别名
- Host Cell Proteins (HCP 产品)
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