Quick Overview for HCP 蛋白 (ABIN1607597)
抗原
HCP
(Host Cell Proteins (HCP))
蛋白类型
Native
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS), Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE), Western Blotting (WB)
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Supplier Product No.
- 000-001-j08
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Supplier
- Rockland
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原理
- E.coli Host Cell Protein Control Protein (HCP) (in 1x Loading Buffer)
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特异性
- E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E. coli (BL21) cells.
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产品特性
- Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: E.coli Host Cell Proteins , HCP antigen, E.coli control antigen
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应用备注
- Western Blot Dilution: 1:500 - 1:1000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.0 mg/mL
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缓冲液
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store Host Cell Proteins control protein at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- HCP (Host Cell Proteins (HCP))
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别名
- Host Cell Proteins
抗原
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