Podoplanin Protein (PDPN) (Soluble) (His tag)
Quick Overview for Podoplanin Protein (PDPN) (Soluble) (His tag) (ABIN1589760)
抗原
See all Podoplanin (PDPN) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- Soluble
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标记
- This Podoplanin protein is labelled with His tag.
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原理
- Podoplanin, soluble
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序列
- GSSHHHHHHS SGLVPRGSHM EGASTGQPED DTETTGLEGV AMPGAEDDVV TPGTSEDRYK SGLTTLVATS VNSVTGIRIE DLPTSESTVH AQEQSPSATA SNVATSHsTE KVDGDTQTTV EKDGLST
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特异性
- Chromosomal location:1p36.21
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产品特性
- Length (aa):127
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Podoplanin (PDPN) protein (Fc Tag)
PDPN 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Podoplanin (PDPN) (AA 99-207) protein (His tag)PDPN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Podoplanin (PDPN) (AA 23-141) protein (His tag)PDPN 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Podoplanin (PDPN) (AA 1-238) protein (GST tag)PDPN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Podoplanin (PDPN) (Active) protein (His tag,Fc Tag)PDPN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Podoplanin (PDPN) protein (His tag)PDPN 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Podoplanin (PDPN) protein (Fc Tag,ECD)PDPN 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
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说明
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Soluble Receptors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.
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缓冲液
- PBS
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注意事项
- Centrifuge vial prior to opening.
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储存条件
- RT,-20 °C
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储存方法
- The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted sPodoplanin should be stored in working aliquots at -20°C.
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- Podoplanin (PDPN)
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别名
- Podoplanin
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背景
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Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosylation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth. The recombinant soluble Podoplanin starts with GLST and ends with GLST.
Synonyms: PDPN, T1A, GP36, GP40, Gp38, OTS8, T1A-2, AGGRUS, HT1A-1, PA2.26 -
分子量
- 13.1 kDa
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基因ID
- 10630
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NCBI登录号
- NM_001006624, NP_001006625
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UniProt
- Q86YL7
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途径
- Dicarboxylic Acid Transport
抗原
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