LIF 蛋白
Quick Overview for LIF 蛋白 (ABIN1589657)
抗原
See all LIF 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- LIF
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序列
- SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
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特异性
- Chromosomal location:11 A1-A2, 11 00.25 cM
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产品特性
- Length (aa):180
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内毒素水平
- < 0.1 ng per μg (IEU/μg) of rm LIF
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Leukemia Inhibitory Factor (LIF) (AA 23-202) protein
LIF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) proteinLIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) protein (Fc Tag)LIF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) protein (His tag)LIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (GST tag)LIF 宿主: 人, 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % (SDS-PAGE) SDS Active
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (Fc Tag)LIF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- The ED50 as determined by the M1 cell differentiation assay is in the range of 0.1 - 0.5 ng/mL.
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The lyophilized LIF should be reconstituted in water to a concentration not less than 100 μg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
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缓冲液
- 0.5X PBS
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储存条件
- RT,0 °C,-20 °C
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储存方法
- The lyophilized LIF, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted LIF should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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- LIF (Leukemia Inhibitory Factor (LIF))
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别名
- LIF
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背景
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Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa.
Synonyms: Lif, leukemia inhibitory factor -
分子量
- 19.86 kDa
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基因ID
- 16878
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NCBI登录号
- NM_008501, NP_032527
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UniProt
- P09056
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
抗原
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