PDGF-BB Homodimer (Homodimer) (Active) 蛋白
(1 reference)Quick Overview for PDGF-BB Homodimer (Homodimer) (Active) 蛋白 (ABIN1589629)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- Homodimer
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原理
- PDGF-BB
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序列
- SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
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特异性
- Chromosomal location:22q13.1
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产品特性
- Length (aa):109
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内毒素水平
- < 0.1 ng per ug of PDGF-BB
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PDGF-BB Homodimer (Active) protein (His tag)
宿主: 人 宿主: 酵母菌 Recombinant > 95 % as determined by reducing SDS-PAGE. Active
PDGF-BB Homodimer protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 98 % as determined by reducing SDS-PAGE.
PDGF-BB Homodimer (AA 82-190) (Active) protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by reducing (R) and non-reducing (NR) SDS-PAGE. Active
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应用备注
- The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. The lyophilized PDGF-BB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.
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缓冲液
- 50 mM acetic acid
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注意事项
- Centrifuge vial prior to opening.
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储存条件
- RT,-20 °C,-80 °C
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储存方法
- The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-BB is best stored at -20°C to -70°C.
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: "Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy." in: Diabetes, Vol. 64, Issue 7, pp. 2581-92, (2015) (PubMed).
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: "Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy." in: Diabetes, Vol. 64, Issue 7, pp. 2581-92, (2015) (PubMed).
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- PDGF-BB Homodimer
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别名
- PDGF-BB
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背景
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PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, INN=Becaplermin -
分子量
- 24.3 kDa
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基因ID
- 5155
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NCBI登录号
- NM_002608, NP_002599
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UniProt
- P01127
抗原
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