PDGF-AA Homodimer (Homodimer) (Active) 蛋白
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for PDGF-AA Homodimer (Homodimer) (Active) 蛋白 (ABIN1589624)
抗原
PDGF-AA Homodimer
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 % by SDS-PAGE
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产品特性
- Homodimer
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原理
- PDGF-AA
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序列
- SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
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特异性
- Chromosomal location:7p22
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产品特性
- Length (aa):125
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内毒素水平
- < 0.1 ng per ug of PDGF-AA
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PDGF-AA Homodimer (AA 87-196) protein (His tag)
宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
PDGF-AA Homodimer protein宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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应用备注
- The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. The lyophilized PDGF-AA should be reconstituted in water to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.
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缓冲液
- 50 mM acetic acid
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注意事项
- Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.
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储存条件
- RT,-20 °C,-80 °C
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储存方法
- The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AA is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.
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- PDGF-AA Homodimer
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别名
- PDGF-AA
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背景
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PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
Synonyms: PDGFA, PDGF1, PDGF-A -
分子量
- 28.5 kDa
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基因ID
- 5154
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NCBI登录号
- NM_002607, NP_002598
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UniProt
- P04085
抗原
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