PLGF Protein (Homodimer)
Quick Overview for PLGF Protein (Homodimer) (ABIN1589565)
抗原
See all PLGF (PGF) 蛋白蛋白类型
生物活性
宿主
资源
纯度
-
-
产品特性
- Homodimer
-
原理
- PlGF
-
序列
- ALSAGNNSTE MEVVPFNEVW GRSYCRPMEK LVYIADEHPN EVSHIFSPSC VLLSRCSGCC GDEGLHCVAL KTANITMQIL KIPPNRDPHS YVEMTFSQDV LCECRPILET TKAERRKTKG KRKQSKTPQT EEPHL
-
特异性
- Chromosomal location:6q31
-
产品特性
- Length (aa):135
-
-
Placenta Growth Factor (PGF) (AA 19-221) protein (Fc Tag)
PGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Placenta Growth Factor (PGF) (Active) proteinPGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Placenta Growth Factor (PGF) (AA 19-158) protein (His tag)PGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Placenta Growth Factor (PGF) (AA 19-158) protein (Fc Tag)PGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Placenta Growth Factor (PGF) (AA 19-170) protein (Fc Tag)PGF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Placenta Growth Factor (PGF) (AA 19-170) (Active) protein (His tag)PGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Placenta Growth Factor (PGF) (AA 19-221) (Active) protein (Fc Tag)PGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Placenta Growth Factor (PGF) protein (His tag)PGF 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Placenta Growth Factor (PGF) (Active) protein (His tag)PGF 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Placenta Growth Factor (PGF) (AA 1-170) protein (His-IF2DI Tag)PGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
-
-
应用备注
- Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant rat PlGF can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.1 - 5 ng/mL
-
说明
-
Cytokines & Growth Factors
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Centrifuge vial prior to opening. The rat PlGF is supplied in lyophilized form and can be reconstituted with water. This solution can be diluted into other buffered solutions or stored frozen for future use.
-
缓冲液
- 25 mM Tris, pH 8.5
-
储存条件
- RT,-20 °C,-80 °C
-
储存方法
- The lyophilized rat PIGF, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C.
-
-
- PLGF (PGF) (Placenta Growth Factor (PGF))
-
别名
- PlGF
-
背景
-
Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF-1), 152 (PlGF-2), and 203 (PlGF-3) amino acids (aa) respectively. Only PlGF-2 contains a highly basic heparin-binding 21 aa insert at the C-terminus. In rat only one PlGF that is the equivalent of human PlGF-2 has been identified. Rat PlGF shares 60 %, 92 %, 62 % and 59% aa identity with the appropriate isoform of human, mouse, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during human pregnancy, reaching a peak in mid-gestation, this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt-1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2-mediated angiogenesis. However, PlGF (especially human PlGF-1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF-2, like VEGF164/165, shows heparin-dependent binding of neuropilin (Npn)-1 and Npn-2 and can inhibit nerve growth cone collapse. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis. Circulating PlGF often correlates with tumor stage and aggressiveness, and therapeutic PlGF antibodies are being investigated to inhibit tumor growth and angiogenesis.
Synonyms: Pgf, Plgf, placental growth factor -
分子量
- 15.14 kDa
-
基因ID
- 94203
-
NCBI登录号
- NM_053595, NP_446047
-
UniProt
- Q63434
-
途径
- VEGFR1 Specific Signals
抗原
-
