PLGF Protein (Homodimer)
Quick Overview for PLGF Protein (Homodimer) (ABIN1589559)
抗原
See all PLGF (PGF) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- Homodimer
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原理
- PlGF-2
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序列
- LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR
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特异性
- Chromosomal location:2p21-p16
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产品特性
- Length (aa):152
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Placenta Growth Factor (PGF) (AA 19-221) protein (Fc Tag)
PGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Placenta Growth Factor (PGF) (Active) proteinPGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Placenta Growth Factor (PGF) (AA 19-158) protein (His tag)PGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Placenta Growth Factor (PGF) (AA 19-158) protein (Fc Tag)PGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Placenta Growth Factor (PGF) (AA 19-170) protein (Fc Tag)PGF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Placenta Growth Factor (PGF) (AA 19-170) (Active) protein (His tag)PGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Placenta Growth Factor (PGF) (AA 19-221) (Active) protein (Fc Tag)PGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Placenta Growth Factor (PGF) protein (His tag)PGF 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Placenta Growth Factor (PGF) (Active) protein (His tag)PGF 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Placenta Growth Factor (PGF) (AA 1-170) protein (His-IF2DI Tag)PGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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应用备注
- Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant human PlGF-2 can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5 - 10 ng/mL.
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. The PlGF-2 is supplied in lyophilized form with carrier-protein (BSA) and can be reconstituted with 50 mM acetic acid or PBS/water. This solution can be diluted into other buffered solutions or stored frozen for future use.
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缓冲液
- 50 mM acetic acid
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储存条件
- RT,-20 °C,-80 °C
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储存方法
- The lyophilized human PIGF-2, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C
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- PLGF (PGF) (Placenta Growth Factor (PGF))
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别名
- PlGF-2
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背景
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Human Placenta Growth Factor-2 (PlGF-2), a 22 kDa protein consisting of 152 amino acid residues is produced as a homodimer. PlGF is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF acts only as a weak mitogen for those cell types possessing receptors for binding (e.g. vascular endothelial cells). At least one high-affinity receptor for PlGF (FLT-1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human umbilical vein endothelial cells and monocytes). In addition to its action as a weak mitogen it is also a chemoattractant for monocytes and endothelial cells. Two different proteins are generated by differential splicing of the human PlGF gene: PlGF-1 (131 aa native chain) and PlGF-2 (152 aa native chain). Both mitogens are secretable proteins, but PlGF-2 can bind to heparin with high affinity. PlGF is apparently a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms posses similar biologcal activities. If PlGF is angiogenic in vivo is not clear. However, heterodimers between VEGF and PlGF are mitogenic for endothelial cells and have strong angiogenic activity in vivo (e.g. in the CAM assay or in the cornea pocket assay). Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A much related protein of PlGF is VEGF with about 53% homology and VEGF-B with similar biological activities.
Synonyms: PlGF, placental growth factor -
分子量
- ~45.0 kDa
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基因ID
- 5281
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NCBI登录号
- NM_001207012, NP_001193941
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UniProt
- P49763
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途径
- VEGFR1 Specific Signals
抗原
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