Protein A Protein (Biotin)
This Protein A protein is expressed in and has been mentioned in 1 publication.
(1 reference)Quick Overview for Protein A Protein (Biotin) (ABIN1536532)
抗原
See all Protein A 蛋白
Protein A
宿主
Staphylococcus aureus
宿主
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应用范围
ELISA, Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC)
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标记
- This Protein A protein is labelled with Biotin.
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Protein A (AA 37-469) protein
宿主: Staphylococcus aureus 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Protein A protein (Biotin)宿主: Staphylococcus aureus Recombinant
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应用备注
- Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:5,000-1:50,000 Western blot: 1:5,000-1:50,000 ELISA: 1:50,000-1:500,000 Other: user-optimized.
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限制
- 仅限研究用
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浓度
- 2 mg/ml
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缓冲液
- PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use.
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: "Specific antibody immobilization with biotin-poly(L-lysine)-g-poly(ethylene glycol) and protein A on microfluidic chips." in: Journal of immunological methods, Vol. 350, Issue 1-2, pp. 97-105, (2009) (PubMed).
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: "Specific antibody immobilization with biotin-poly(L-lysine)-g-poly(ethylene glycol) and protein A on microfluidic chips." in: Journal of immunological methods, Vol. 350, Issue 1-2, pp. 97-105, (2009) (PubMed).
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- Protein A
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背景
- Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. The product contains five high-affinity binding sites capable of interacting with the Fc region of IgG in humans, rabbits, pigs, and other species. Biotin-Protein A, which is produced by covalently linking biotin to protein A, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
抗原
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