CDNF 蛋白
This Recombinant CDNF protein is expressed in CHO Cells.
Quick Overview for CDNF 蛋白 (ABIN1405917)
抗原
See all CDNF 蛋白
CDNF
(Cerebral Dopamine Neurotrophic Factor (CDNF))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
CHO Cells
应用范围
Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)
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产品特性
- Biological properties: Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007, Voutilainen et al. 2011)
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纯化方法
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Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Protein is sterile-filtrated through 0.22 µg filter.
Purified human CDNF is characterized as homogenous material by mass-spectroscopy. -
内毒素水平
- Less than 1EU/µg of protein as determined by LAL method
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Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 25-187) protein (His tag)
CDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cerebral Dopamine Neurotrophic Factor (CDNF) protein (His tag)CDNF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
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说明
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Synonyms: Cerebral dopamine neurotrophic factor - CDNF, ARMETL1.
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限制
- 仅限研究用
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浓度
- 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
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缓冲液
- PBS pH 7.5
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注意事项
- Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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- CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))
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别名
- CDNF
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背景
- CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007, Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
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分子量
- Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
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UniProt
- Q49AH0
抗原
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