TNFSF13 Protein (AA 16-108, AA 88-233) (DYKDDDDK Tag)
(9 references)-
- 抗原 See all TNFSF13 蛋白
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 16-108, AA 88-233
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宿主
- 人, 小鼠
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资源
- HEK-293 Cells
- 标记
- This TNFSF13 protein is labelled with DYKDDDDK Tag.
- 应用范围
- SDS-PAGE (SDS)
- 原理
- APRIL (human) (multimeric) (rec.)
- 品牌
- MegaAPRIL™
- 特异性
- Binds to human and mouse BCMA and TACI. Binds to proteoglycans.
- 交叉反应
- 人, 小鼠
- 产品特性
- The extracellular domain of human APRIL (aa 88-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG®-tag.
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.01EU/μg purified protein (LAL test).
- Biological Activity Comment
- Stimulates B cell proliferation.
- Top Product
- Discover our top product TNFSF13 蛋白
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Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 112-250), (Trimer) protein (His-DYKDDDDK Tag)
TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 96-241) protein (Fc Tag)TNFSF13 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 112-250), (Trimer) protein (His-Avi-DYKDDDDK Tag)TNFSF13 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 1-247) protein (GST tag)TNFSF13 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 111-250) (Active) protein (His tag,DYKDDDDK Tag)TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-250) (Active) protein (Fc Tag,AVI tag,Biotin)TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 112-250), (Trimer) protein (His-Avi-DYKDDDDK Tag,Biotin)TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) protein (Fc Tag)TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- 0.1 mg/mL
- 缓冲液
- Contains PBS.
- 注意事项
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- 有效期
- 6 months
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: "STAT-3 activation by differential cytokines is critical for human in vivo-generated plasma cell survival and Ig secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 10, pp. 4996-5004, (2013) (PubMed).
: "TACI regulates IgA production by APRIL in collaboration with HSPG." in: Blood, Vol. 109, Issue 7, pp. 2961-7, (2007) (PubMed).
: "BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway." in: Blood, Vol. 109, Issue 2, pp. 703-10, (2007) (PubMed).
: "APRIL and BAFF promote increased viability of replicating human B2 cells via mechanism involving cyclooxygenase 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 11, pp. 6736-51, (2006) (PubMed).
: "BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway." in: The Journal of experimental medicine, Vol. 202, Issue 10, pp. 1363-74, (2005) (PubMed).
: "Potential role of APRIL as autocrine growth factor for megakaryocytopoiesis." in: Blood, Vol. 104, Issue 10, pp. 3169-72, (2004) (PubMed).
: "Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 5, pp. 3268-79, (2004) (PubMed).
: "DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL." in: Nature immunology, Vol. 3, Issue 9, pp. 822-9, (2002) (PubMed).
: "APRIL modulates B and T cell immunity." in: The Journal of clinical investigation, Vol. 109, Issue 12, pp. 1587-98, (2002) (PubMed).
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: "STAT-3 activation by differential cytokines is critical for human in vivo-generated plasma cell survival and Ig secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 10, pp. 4996-5004, (2013) (PubMed).
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- 抗原
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
- 别名
- APRIL (TNFSF13 产品)
- 背景
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MultimericAPRIL™, ACRP30headless:APRIL, ACRP30headless:CD256, ACRP30headless:TNFSF13, ACRP30headless:A-proliferation-inducing Ligand:
APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the NH2 terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.
- 分子量
- ~36kDa (SDS-PAGE)
- UniProt
- O75888
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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