(6 references)-
- 抗原 See all EPO 蛋白
- EPO (Erythropoietin (EPO))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func)
- 品牌
- BD Pharmingen™
- 特异性
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Biological Activity Measured using TF-1 indicator cells
Specific Activity: 0.1 - 1.0 x 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation).
ED50: 0.1 - 1.0 ng/ml
Observed linear dose response range: >100 fold - 产品特性
- Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, and containing 1 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant mouse EPO is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.
- 纯化方法
- The recombinant mouse EPO is > 95 % pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of mouse EPO, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product EPO 蛋白
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Erythropoietin (EPO) (AA 28-193) (Active) protein
EPO 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Erythropoietin (EPO) (AA 1-193) protein (GST tag)EPO 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Erythropoietin (EPO) (Active) protein (His tag)EPO 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Erythropoietin (EPO) (AA 27-192) protein (His tag)Crystallography grade EPO 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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BD Pharmingen™ Mouse Erythropoietin Recombinant Protein - Reactivity Ms
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1 mg/mL
- 缓冲液
- Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, and containing 1 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.
- 储存液
- Azide free
- 注意事项
- Rapidly thaw and quick-spin product prior to use. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
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: "Nucleotide sequence of rat erythropoietin." in: Biochimica et biophysica acta, Vol. 1171, Issue 1, pp. 99-102, (1992) (PubMed).
: "Erythropoietin." in: Blood, Vol. 77, Issue 3, pp. 419-34, (1991) (PubMed).
: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).
: "Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 842-8, (1986) (PubMed).
: "Murine erythropoietin gene: cloning, expression, and human gene homology." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 849-58, (1986) (PubMed).
: "Role of the kidney in erythropoiesis." in: Nature, Vol. 179, Issue 4560, pp. 633-4, (1957) (PubMed).
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: "Nucleotide sequence of rat erythropoietin." in: Biochimica et biophysica acta, Vol. 1171, Issue 1, pp. 99-102, (1992) (PubMed).
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- 抗原
- EPO (Erythropoietin (EPO))
- 别名
- EPO (EPO 产品)
- 别名
- EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein
- 物质类
- Hormone
- 背景
- Mouse erythropoietin (EPO) is a 30 kD heavily glycosylated protein containing 166 amino acids. The carbohydrate residues compose approximately 30 % of the molecule by weight.3 It shares 80 % and 95 % homology with human and rat EPO, respectively. EPO functions as the survival and proliferation factors of late erythroid progenitor cells (CFU-E). In adult mammals, EPO is synthesized almost exclusively in the kidneys.
- 途径
- JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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