(4 references)-
- 抗原 See all IL-4 (IL4) 蛋白
- IL-4 (IL4) (Interleukin 4 (IL4))
- 蛋白类型
- Recombinant
-
宿主
- 大鼠
-
资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 特异性
- Biological Activity: This material has been shown to have biological activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
- 产品特性
- Recombinant rat IL-4 is supplied as a frozen liquid comprised of 0.22 ìm sterile-filtered PBS, pH 7.2, and containing 2.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat IL-4 protein was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.
- 纯化方法
- Recombinant rat IL-4 protein was purified by immunoaffinity chromatography and was found to be > 95 % pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of rat IL-4, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product IL4 蛋白
-
Interleukin 4 (IL4) (AA 25-153) protein
IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His tag)IL4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) protein (His-Avi Tag)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 23-140) (Active) proteinIL4 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) proteinIL4 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 4 (IL4) (AA 22-147) protein (His tag)IL4 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 4 (IL4) (AA 25-153) protein (His-IF2DI Tag)IL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 4 (IL4) protein (His tag)IL4 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
-
- 应用备注
-
ELISA Standard: Recombinant rat IL-4 (Cat. No. 555107) is useful as a quantitative standard for measuring rat IL-4 protein levels in an IL-4 specific sandwich ELISA with purified anti-rat IL-4, clone OX-81, (Cat. No. 555080) as a capture antibody and biotinylated anti-rat IL-4, clone B11-3 (Cat. No. 555090) as the detection antibody. To obtain linear standard curves, doubling dilutions of the rat IL-4 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring rat IL-4 in serum or plasma our BD OptEIA™ Rat IL-4 ELISA Set (Cat. No. 555198) is specially formulated and recommended. - 说明
-
BD Pharmingen™ Recombinant Rat IL-4
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Recombinant rat IL-4 is supplied as a frozen liquid comprised of 0.22 im sterile-filtered PBS, pH 7.2, and containing 2.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.
- 储存液
- Azide free
- 注意事项
-
This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Recombinant rat IL-4 is supplied as a frozen liquid. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml.
Note: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. - 储存条件
- -80 °C
- 储存方法
- This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Recombinant rat IL-4 is supplied as a frozen liquid. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein** such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. NOTE: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
-
-
: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4." in: Immunology, Vol. 75, Issue 2, pp. 286-92, (1992) (PubMed).
: "Molecular cloning of rat interleukin 4 cDNA and analysis of the cytokine repertoire of subsets of CD4+ T cells." in: European journal of immunology, Vol. 21, Issue 5, pp. 1187-94, (1991) (PubMed).
: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, (1991) (PubMed).
-
: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
-
- 抗原
- IL-4 (IL4) (Interleukin 4 (IL4))
- 别名
- IL-4 (IL4 产品)
- 别名
- IL4 Protein, IL-4 Protein, BCGF-1 Protein, BCGF1 Protein, BSF-1 Protein, BSF1 Protein, Il-4 Protein, Il4e12 Protein, IL2 Protein, interleukin 4 Protein, IL4 Protein, Il4 Protein
- 背景
- Interleukin-4 (IL-4) is a potent lymphoid cell growth factor which can stimulate the growth, differentiation and survivability of and regulate the function of various cell types of hematopoietic (including B and T lymphocytes, mast cells, monocytes and macrophages) and non-hematopoietic (e.g., vascular endothelial cells and certain tumor cells) origin. IL-4 exerts its biological activities by binding to specific high affinity receptors expressed by a wide variety of cell types. The rat IL-4 gene product is a 17 -20 kD glycoprotein containing 123 amino acid residues.
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
-
You are here:
