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- 抗原 See all IL-4 (IL4) 蛋白
- IL-4 (IL4) (Interleukin 4 (IL4))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 特异性
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Biological Activity: Proliferation is measured using MC/9 indicator cells pulsed with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide).
Specific Activity: 0.3 - 2.0 x 10^8 Units/mg
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.
ED50: 50 - 300 pg/mL - 产品特性
- Recombinant mouse IL-4 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, pH 7.2, containing glycerol and 2.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-4 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers Lambert Law.
- 纯化方法
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Immunoaffinity Chromatography
Recombinant mouse IL-4 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IL-4 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. - 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of mouse IL-4, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product IL4 蛋白
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- 应用备注
- ELISA Standard: Mouse IL-4 is useful as a quantitative standard for measuring mouse IL-4 protein levels in an IL-4 specific sandwich ELISA with the purified 11B11 antibody (Cat. No. 554434) as a capture antibody and the biotinylated BVD6-24G2 (Cat. No. 554390) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-4 standard from ~2,000 to 15 pg/mL should be included in each ELISA plate. For assay of mouse IL-4 in complex fluids such as serum or plasma, the BD OptEIA™ Mouse IL-4 ELISA Set is recommended (Cat. No. 555198).
- 说明
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BD Pharmingen™ Recombinant Mouse IL-4 - Reactivity Ms
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA and glycerol.
- 储存液
- Azide free
- 注意事项
- Interleukin-4 (IL-4) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes. IL-4 is responsible for activities previously assigned to BCGF, BCDF, BSF-2 and TCGF-2. Mouse IL-4 is a 14 kD protein containing 120 amino acid residues.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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- 抗原
- IL-4 (IL4) (Interleukin 4 (IL4))
- 别名
- IL-4 (IL4 产品)
- 别名
- IL4 Protein, IL-4 Protein, BCGF-1 Protein, BCGF1 Protein, BSF-1 Protein, BSF1 Protein, Il-4 Protein, Il4e12 Protein, IL2 Protein, interleukin 4 Protein, IL4 Protein, Il4 Protein
- 背景
- Interleukin-4 (IL-4) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes. IL-4 is responsible for activities previously assigned to BCGF, BCDF, BSF-2 and TCGF-2. Mouse IL-4 is a 14 kD protein containing 120 amino acid residues.
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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