(4 references)-
- 抗原
- CTLA-4 IgG Fusion
- 宿主
- 小鼠
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- 应用范围
- Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 纯化方法
- Purified from tissue culture supernatant or ascites by affinity chromatography
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤0.01 ng/μg of protein.
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- 应用备注
- This fusion protein has been tested by LAL assay for endotoxin level, by SDS-PAGE to assure purity, and by an in vitro T-cell proliferation assay to assure blocking activity.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Avoid multiple freeze-thaws of product. The fusion protein solution should be stored at -20°C until the vial is opened. Thawed aliquots may be stored for at least 1 week at 4°C.
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: "The B7 family of ligands and its receptors: new pathways for costimulation and inhibition of immune responses." in: Annual review of immunology, Vol. 20, pp. 29-53, (2002) (PubMed).
: "CTLA4Ig prevents lymphoproliferation and fatal multiorgan tissue destruction in CTLA-4-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5091-4, (1997) (PubMed).
: "CD28/B7 system of T cell costimulation." in: Annual review of immunology, Vol. 14, pp. 233-58, (1996) (PubMed).
: "Ex vivo coating of islet cell allografts with murine CTLA4/Fc promotes graft tolerance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1165-74, (1995) (PubMed).
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: "The B7 family of ligands and its receptors: new pathways for costimulation and inhibition of immune responses." in: Annual review of immunology, Vol. 20, pp. 29-53, (2002) (PubMed).
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- 抗原
- CTLA-4 IgG Fusion
- 物质类
- Antibody
- 背景
- The Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein is composed of the extracellular domain of mouse CTLA-4 (CD152) fused to a mutant Fc region of mouse IgG2a, which is unable to bind to the C1q complement or to Fc receptors. This chimeric protein binds to CTLA-4's ligands B7-1 (CD80) and B7-2 (CD86) on mouse antigen-presenting cells and blocks their binding to both CTLA-4 and CD28. CTLA-4 (also known as CD152) is a cell-surface Ig-superfamily glycoprotein closely related to the CD28 costimulatory receptor. CTLA-4 is expressed on activated T lymphocytes 2-3 days after stimulation through the T-cell receptor. Whereas CD28 delivers a costimulatory signal required for T-cell activation, CTLA-4 is a negative regulator of cell-mediated immune responses. CTLA-4 IgG Fusion Protein has been shown to prevent allograft rejection and induce donor-specific tolerance, inhibit in vitro responses of splenocytes to ConA, inhibit the spontaneous in vitro and in vivo lymphoproliferation in CTLA-4-deficient mice, and limit superantigen-induced T-cell proliferation, anergy, and secretion of IL-2, IFN-gamma, and IL-4, but not TNF-alpha or IL-10. Inhibition of ConA-induced proliferation of splenic T lymphocytes by Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein. C57BL/6 splenocytes were cultured for three days, either with no stimulation or with 4 µg ConA per 10^6 cells, as indicated. The indicated concentrations of either Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein or a control fusion protein were added to ConA-stimulated cells. Cell proliferation was quantitated by the MTT fluorometric assay. The CTLA-4 fusion protein inhibited ConA-induced cell proliferation in a dose-dependent manner, whereas the control fusion protein had little effect.
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