CD34 Protein (CD34)
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北京 101111
Quick Overview for CD34 Protein (CD34) (ABIN1111306)
抗原
See all CD34 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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原理
- Human CD34 Protein Recombinant
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产品特性
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Protein Size: Recombinant CD34 is a monomer protein consisting of 270 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human extracellular domain of human CD34 including a C-terminal 6His tag was expressed in HEK293 cells.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Measured by the ability to support the adhesion of the HUVEC cell line. Coated plates with immobilized recombinant Human CD34 ( 0.8 μg/mL) are able to adhere 40% of cells ( 4 x 104 cells/well) after one hour at 37 ℃
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CD34 (CD34) (AA 32-290) protein (His tag)
CD34 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
CD34 (CD34) (AA 35-287) (Active) protein (His tag)CD34 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
CD34 (CD34) (Active) protein (His tag)CD34 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
CD34 (CD34) protein (Fc Tag)CD34 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
CD34 (CD34) (AA 35-287) protein (Fc Tag)CD34 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
CD34 (CD34) (AA 32-141) protein (GST tag)CD34 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
CD34 (CD34) (AA 1-328) protein (GST tag)CD34 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
CD34 (CD34) (AA 124-225) protein (His tag)CD34 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CD34 (CD34) (AA 32-290) protein (His-IF2DI Tag)CD34 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CD34 (CD34) (AA 32-290) protein (His tag)CD34 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % SDS
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限制
- 仅限研究用
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溶解方式
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
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缓冲液
- Recombinant CD34 is supplied as a 0.2 μm filtered PBS solution, pH 7.2.
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储存条件
- -20 °C
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储存方法
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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有效期
- 24 months
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- CD34
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别名
- CD34
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分子量
- 50 kDa
抗原
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