VEGF 165 (AA 207-371) 蛋白
Quick Overview for VEGF 165 (AA 207-371) 蛋白 (ABIN1098802)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 207-371
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序列
- MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
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纯化方法
- purified by chromatography
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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VEGF 165 (AA 27-191) protein
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) protein (His-Avi Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 207-371) protein (His tag)宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
VEGF 165 (AA 27-191) protein (His tag)宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 85% by SDS-PAGE SDS
VEGF 165 protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA, 0.1 MM PMSF, 1 MM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- VEGF 165
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背景
- Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
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分子量
- 19.3 kDa (166aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_001020539
抗原
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