MDH protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for MDH protein (His tag) (ABIN1098249)
抗原
MDH
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 95 % by SDS - PAGE
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标记
- His tag
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纯化方法
- purified by chromatography
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MDH (AA 2-312) protein (His tag)
宿主: Bacillus subtilis 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
MDH (AA 1-312), (full length) protein (His tag)宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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说明
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Synonyms: malate dehydrogenase, ECK3225, JW3205
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/ml (determined by Bradford assay)
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缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl
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储存条件
- 4 °C
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储存方法
- Avoid repeated freezing and thawing cycles.
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- MDH
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背景
- Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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分子量
- 34.9kDa (336aa), confirmed by MALDI-TOF
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NCBI登录号
- NP_417703
抗原
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