ESAT6 (AA 1-95) protein (His tag)
This Recombinant protein is expressed in Baculovirus infected Insect Cells.
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Quick Overview for ESAT6 (AA 1-95) protein (His tag) (ABIN7676528)
抗原
ESAT6
蛋白类型
Recombinant
宿主
Mycobacterium tuberculosis
资源
Baculovirus infected Insect Cells
应用范围
SDS-PAGE (SDS)
纯度
> 90 %
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产品特性
- AA 1-95
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标记
- His tag
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序列
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MTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFA -
纯化方法
- > 90% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Synonyms: Early secretory antigenic target, 6 kDa
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ESAT6
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背景
- ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.
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分子量
- 11 kDa (104aa)
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NCBI登录号
- NP_338543
抗原
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