NAGK Protein (AA 1-344) (His tag)
Our Local Distributor
北京 101111
Quick Overview for NAGK Protein (AA 1-344) (His tag) (ABIN1098243)
抗原
See all NAGK 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 1-344
-
标记
- This NAGK protein is labelled with His tag.
-
序列
-
MAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS -
纯化方法
- > 95% by SDS-PAGE
-
-
N-Acetylglucosamine Kinase (NAGK) (AA 1-344) protein (His-IF2DI Tag)
NAGK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
N-Acetylglucosamine Kinase (NAGK) (AA 1-344) protein (GST tag)NAGK 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
N-Acetylglucosamine Kinase (NAGK) (AA 2-344) protein (His-SUMO Tag)NAGK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
N-Acetylglucosamine Kinase (NAGK) protein (Myc-DYKDDDDK Tag)NAGK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Synonyms: N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 2 mM DTT, 20 % glycerol, 200 mM NaCl
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
-
-
- NAGK (N-Acetylglucosamine Kinase (NAGK))
-
别名
- N-Acetyl-D-Glucosamine Kinase/NAGK
-
背景
- NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity. Recombinant human NAGK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
-
分子量
- 39.8 kDa (367aa) confirmed by MALDI-TOF
-
NCBI登录号
- NP_060037
抗原
-
