IGF-r3 (AA 49-118) (Active) 蛋白
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for IGF-r3 (AA 49-118) (Active) 蛋白 (ABIN1097099)
抗原
IGF-r3
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Functional Studies (Func)
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 49-118
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原理
- Recombinant Human LR3 Insulin-Like Growth Factor I/LR3-IGF-1 (MG)
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序列
- MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
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产品特性
- Recombinant Human LR3-IGF-1 (MG) is produced with our E. coli expression system. The target protein is expressed with sequence (G49-A118) of Human IGF1 R3.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.05 ng/μg (0.5 IEU/μg) as determined by LAL test
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说明
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Biological activity: ED50 is greater than 200 ng/ml as determined by an IGF binding protein assay.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in 500 mM Acetic Acid.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 300 mM HAc-NaAc, pH 6.5.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
有效期
- 3 months
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- IGF-r3
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背景
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Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF-1 binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-I in vitro.
Alternative Names: Insulin-Like Growth Factor I, IGF-I, Mechano Growth Factor, MGF, Somatomedin-C, IGF1, IBP1 -
分子量
- 9.1 kDa
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UniProt
- P05019
抗原
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