IGF-r3 (AA 49-118) (Active) 蛋白
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Func
Active
-
- 抗原
- IGF-r3
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 49-118
- 宿主
- 人
- 资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func)
- 原理
- Recombinant Human LR3 Insulin-Like Growth Factor I/LR3-IGF-1 (MG)
- 序列
- MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
- 产品特性
- Recombinant Human LR3-IGF-1 (MG) is produced with our E. coli expression system. The target protein is expressed with sequence (G49-A118) of Human IGF1 R3.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.05 ng/μg (0.5 IEU/μg) as determined by LAL test
-
- 说明
-
Biological activity: ED50 is greater than 200 ng/ml as determined by an IGF binding protein assay.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in 500 mM Acetic Acid.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 300 mM HAc-NaAc, pH 6.5.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
-
- 抗原
- IGF-r3
- 背景
-
Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF-1 binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-I in vitro.
Alternative Names: Insulin-Like Growth Factor I, IGF-I, Mechano Growth Factor, MGF, Somatomedin-C, IGF1, IBP1 - 分子量
- 9.1 kDa
- UniProt
- P05019
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