LIF Protein (AA 23-202)
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北京 101111
Quick Overview for LIF Protein (AA 23-202) (ABIN1690795)
抗原
See all LIF 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 23-202
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原理
- Recombinant Human Leukemia Inhibitory Factor/LIF
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序列
- MSPLPITPVN ATCAIRHPCH NNLMNQIRSQ LAQLNGSANA LFILYYTAQG EPFPNNLDKL CGPNVTDFPP FHANGTEKAK LVELYRIVVY LGTSLGNITR DQKILNPSAL SLHSKLNATA DILRGLLSNV LCRLCSKYHV GHVDVTYGPD TSGKDVFQKK KLGCQLLGKY KQIIAVLAQA F
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产品特性
- Recombinant Human Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser23-Phe202 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein
LIF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) proteinLIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) (Active) protein (hFc Tag)LIF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) protein (His tag)LIF 宿主: 小鼠 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF 宿主: 人 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (Fc Tag)LIF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
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说明
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Biological activity: ED50 is less than 0.01 ng/ml as determined by the M1 cell differentiation assay.Specific Activity of 1.0 x 108 IU/mg.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
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储存条件
- 4 °C,-20 °C
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储存方法
- Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 3 weeks
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- LIF (Leukemia Inhibitory Factor (LIF))
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别名
- leukemia-inhibitory-factor
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背景
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Alternative Names: Leukemia Inhibitory Factor, LIF, Differentiation-Stimulating Factor, D Factor, Melanoma-Derived LPL Inhibitor, MLPLI, Emfilermin, LIF, HILDALeukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Alternative Names: Recombinant Human LIF, Leukemia Inhibitory Factor, LIF, Differentiation-Stimulating Factor, D Factor, Melanoma-Derived LPL Inhibitor, MLPLI, Emfilermin, LIF, HILDA -
分子量
- 19.7 kDa
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UniProt
- P15018
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
抗原
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