ED50 is approximately 2 ng/mL as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts. Specific Activity of 5 x 10 5 IU/mg.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
IL17A
宿主: 人
宿主: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, pH 7.4.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Recombinant Human Interleukin-17A/IL-17A produced in E. coli is a single non-glycosylated polypeptide chain containing 133 AAs with a molecular mass of 15,256 Daltons. Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As IL-17 shares properties with IL-1 and TNF-α, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. IL-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation.